Isolate and presumptively identify enterococci / Group D streptococci with Thermo Scientific™ Oxoid™ Bile Aesculin Agar (Dehydrated). Heat to boiling to dissolve the medium completely. Preparation Instructions Suspend 63.5 grams of Bile Esculin Agar Base in 1000 mls of distilled water. Meyer and Schonfeld incorporated bile into the esculin medium and showed that 61 of 62 enterococci were able to grow and split esculin, whereas the other streptococci could not. II PERFORMANCE TEST PROCEDURE 1. Add 1 gram of E8250 Esculin and mix. Dispense in tubes or erlenmeyer flasks. Cool to 45-50°C and pour the mediia from the erlenmeyer flasks into plates. Sterilize by autoclaving at 15 lbs. V SUMMARY AND EXPLANATION Rochaix noted the value of esculin hydrolysis in the identification of enterococci.1 The enterococci were able to split esculin, but other streptococci could not. Meyer and Schonfeld incorporated bile into the esculin medium and showed … Bile esculin agar (BEA) is a differential and selective medium and is mainly used to discern group D Streptococci and Enterococci based on the organism’s potential to hydrolyze esculin. BD BBL™ Bacteroides Bile Esculin Agar is a selective medium for the isolation and presumptive identification of the group. Bile Esculin Azide Agar is a modification of the medium reported by Isenberg and Isenberg, Goldberg, and Sampson. Reduce all BBE plates overnight at room temperature in a BD GasPak™ EZ anaerobic system. pressure (121°C) for 15 minutes. However, other tests such as salt tolerance should be performed for identifying Enterococci ( 3). Swan used an esculin medium containing 40% bile salts and reported that a positive reaction on the bile esculin medium correlated with a serological group D precipitin reaction. This formula modifies Bile Esculin Agar by adding sodium azide and reducing the concentration of bile. Bacteroides Bile Esculin Agar is a selective medium for the isolation and presumptive identification of the B. fragilisgroup. Bile Aesculin Agar is designed to differentiate between Group D streptococci and non-Group D streptococci.. Group D streptococci hydrolyse aesculin to form aesculetin and dextrose.Aesculin hydrolysis is indicative of a positive result. Bile Esculin Agar was also shown to aid differentiation of Enterobacteriaceae, Klebsiella, Enterobacter, Serratia from other Enterobacteriaceae genera (11) on the basis of esculin hydrolysis. Preparation of inocula a. Preparation of inocula a. The medium is highly nutritious. The revised medium is more selective, but still provides rapid growth and efficient recovery of group D streptococci. 2. Intended use The selective isolation and differentiation of enterococci. II PERFORMANCE TEST PROCEDURE 1. 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