To estimate the maximum usable magnification, multiply the aperture (in inches) by 50. The calculator determines the magnification of an optical microscope with a digital camera when a specimen image is displayed on a monitor. As an example, let us calculate the size of a specimen that will be recorded on the 1/2" (6.4 × 4.8 mm) camera sensor if a microscope objective lens magnification is 40 and the camera relay lens magnification is 0.35: Beyond this magnification, objects will continue to look bigger, but they won’t look better. M = D × 0.25 + 1. where M is the magnification, D is the optical power in diopters and 0.25 is the reference distance in meters. To calculate the magnification of an optical microscope, use our Microscope Magnification Calculator. Several formulas are used to determine magnification from the optical power in diopters, but the most common are only two: M = D × 0.25. Some of the tools are perfectly accurate and straightforward such as the stacked lens magnification calculator, however others such as an extension tube magnification calculator rely upon knowledge of the lens macro ratio and its optical centre. Example: Calculate the magnification of a digital microscope with a 45× objective lens, 10× ocular, 0.35× camera adapter lens with a 2-megapixel 1/2.5” (5.76 × 4.29 mm) sensor with resolution 1600 × 1200 pixels on a 32-inch 2560x1440 monitor. Wide-field 10× eyepieces with field number 20 mm and 16 mm. where H sens is the camera sensor height in mm and M total cam is the total camera magnification determined above. Notes: 1: Atmospheric seeing conditions (the sky) often limits the maximum usable magnification to 250-350x. If you’re using millimeters, multiply the aperture by 2. Definitions and Formulas . At the end of the calculator description, we will provide a review of a Miko India microscope and tell you how NOT to buy a microscope using this microscope as an example. Calculating the maximum usable magnification. The formula used to calculate total magnification on the TV monitor is: Total Magnification = Objective Magnification x Microscope Adapter Magnification x Video Magnification. Newtonian telescopes). In fact, they’ll start to look much worse. 2: An exit pupil size (diameter of light beam as it exits eyepiece) over 7.5mm might be too large for telescope designs with central obstructions (i.e. A reference chart is given below which shows video magnification for various monitor and chip sizes. Every telescope has a maximum usable magnification. Magnification Useful calculators and formulae. The video magnification is determined by dividing the TV monitor diagonal (mm) by the CCD chip diagonal (mm).
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