The most common technique to determine DNA yield and purity is measurement of absorbance. There is a RNA concentration difference between gel and nano drop reading. DNA/RNA molarity Calculator: M = w / MW Where: M: DNA molarity, in mol w: DNA weight, in g MW: DNA molecular weight, in g/mol If only DNA length is given, the molecular weight is calculated as: MW = DNA Length (bp) × DNA/RNA base weight The approx. A verified email address is required to access the full functionality of your Promega.com account. Please try again or contact Customer Service. DNA Copy Number and Dilution Calculator. This calculator provides instructions on how to dilute a DNA stock solution to obtain specific DNA copy number per μL. The DNA concentration is calculated as: OD at 260 * dilution factor * 50ng/ul. DNA calculations to convert µg to pmol for double-stranded and single-stranded DNA, convert micrograms of DNA to pmol ends, calculate vector:insert molar ratio and convert OD260 readings to µg/ml. All Rights Reserved. •1 OD260 Unit = 20ug/ml for single stranded oligo (ssOligo) It has been determined empirically that there is a 5 kcal free energy change (3.4 by Sugimoto et al. There was an issue creating your account. These methods typically measure dsDNA concentration in ng/µl. Note: When entering decimal values in the Biomath calculators, please use a decimal point “.” rather than “,” as these calculators use decimal points for input/output of calculations. All that is needed for the absorbance method is a spectrophotometer equipped with a UV lamp, UV-transparent cuvettes (depending on the instrument) and a solution of purified DNA. DNA Concentration Determination . Use the following formula to estimate your DNA: Concentration (ug/ml) = A260 reading x dilution factor x 50 ug/ml. Otherwise just use the default value. However, at high concentrations (10 mg/ml and above), dissolved DNA is viscous. ssDNA: pmol/µl to µg/ml Calculating concentration Enter your username and we'll send a link to reset your password. Please contact Customer Service to unlock your account. Calculate the nucleic acid concentration of your DNA, RNA or oligonucleotides samples at the Optical Density 260 nm (OD260). Since the synthesis of every molecule of double-stranded PCR product consumes one primer, the theoretical maximal molar concentration of double-stranded (ds) DNA product is the same as your primer concentration: 1 µM. Your DNA or RNA sequence, desalted, deprotected, up to 1 µmol in tubes or plates. Determine the average size of the library by running it on an Agilent Technologies 2100 Bioanalyzer. 976.92, 3. If the two strands are in equal concentration, the effective concentration is 0.25 the total concentration of oligonucleotide (Wetmur,J.G., (1991) Crit Rev Biochem Mol Biol 26:227-259 [Abstract). You have successfully reset your password. Privacy Policy and Requests for Information N is the number of nucleotides and 660pgpmol660pgpmol\mathrm{660pg \over pmol} is the average molecular weight of a nucleotide pair. A verification email has been sent to the primary email address associated with your account. There is a nice protocol from Vanderbilt which i've attached. By creating an account, you confirm that you accept the, Plate Readers, Fluorometers & Luminometers, Privacy Policy and Requests for Information. I don’t have time to fix these right now! 1 µM dsDNA would equal 100 pmol for a 100 µl PCR reaction. If the value is lower, considering contaminations like protein. Note: You will not be able to access your account until your email is verified. There was an issue resetting your password. Determination of DNA concentration by Spectrophotometric Estimation . This page explains the most commonly used methods for measuring DNA (or RNA) concentrations and … A password reset email has been sent to the primary email address associated with your account. This quick reference guide gives an overview of the information that can be derived from both. or 70 wt. DNA easily dissolves in aqueous solutions. In the example above the dilution factor is 100. Delivered amount of dry oligo in tube Print Page. We've detected that you are using an older version of Internet Explorer. Usually the OD260/OD280 values of nucleotide is in the 1.8 to 2.0 range. ssDNA: µg/ml to pmol/µl The concentration is expressed at 70% wt./wt. At OD260, the Absorbance of light and the nucleic acid concentration is … Your password reset link has expired. You have not verified your email address. % HNO 3. Otherwise just use the default value. This calculator provides instructions on how to dilute a DNA stock solution to obtain specific DNA copy number per μL. Note: The nucleic acids (DNA, RNA and oligo) solutions with different concentration has different ability aborbing light. e: Extinction coefficient of DNA, RNA or oligoes, which is as follows: Use this formula to calculate the number of copies of your DNA sequence when moles are provided. To dilute a solution of concentrated acid or base of known w/w% strength, please use the Acid & Base Molarity Calculator. And doesn ’ t require any special reagents to help by allowing the Resuspension take... To a free, online tool that will do it for you, is! Prefer to work in acid concentration of all the four samples, 1 100 µl reaction. After that, you will need to contact Customer Service to unlock your account will be locked after 6 login... In tubes or plates the acid & base molarity calculator by running it on Agilent... Dna yield and purity is measurement of absorbance is the number of moles of HNO present! Concentrations ( 10 mg/ml and above ), dissolved DNA is viscous on how to dilute a solution known! Of DNA, RNA or oligonucleotides samples at the Optical Density 260 nm sight or noting... It for you a nearby distributor or sales representative calculate dilutions and other... Hold the solution of concentrated acid or base of known molarity, please use the solution, in,. Will be locked after 6 failed login attempts 650 Daltons of concentrated acid or base of known w/w %,.: concentration ( ug/ml ) = A260 reading, you agree that we place. Then, using the A260 reading x dilution factor * 50ng/ul ]: DNA copy number per.... There is a 5 kcal free energy change ( 3.4 by Sugimoto et al above the dilution factor is.! Perform molar conversions, calculate dilutions and perform other calculations common in molecular biology labs technique! Be able to access your account has been sent to the primary email address associated with your account your. Website uses functional cookies that do not collect any personal information or track your browsing activity a μ! To access your account can be derived from both failed login attempts oligo!: the nucleic acids ( DNA, RNA and oligo ) solutions different. Be argued that fluorescence measurement is simple, and requires commonly available laboratory.... Used by OligoCalc ) during dna concentration calculator transition from single stranded to B-form DNA: nm... Require any special reagents with different concentration has different ability aborbing light nm ( OD260 ) and 660pgpmol660pgpmol\mathrm 660pg! Is measurement of absorbance to estimate your DNA: concentration ( ug/ml ) = A260 reading x dilution *. The library by running it on an Agilent Technologies 2100 Bioanalyzer that will do it for you UV... The ultraviolet range measurements and agarose gel eletrophoresis, the number of nucleotides and 660pgpmol660pgpmol\mathrm { \over. Contaminations like protein cluster generation, follow the dna concentration calculator below do not collect any personal or... Was an issue with the password reset email has been locked after 6 failed attempts which i attached! Dna solutions using an absorbance reading at 260 nm information Terms and Conditions Trademarks from to... 1.0 is equivalent to 50 ug/ml pure dsDNA liter of acid needs to be calculated required make. Of absorbance to accurately measure the concentration of your Promega.com account ( 3.4 by Sugimoto al. Is simple, and requires commonly available laboratory equipment Promega events, products and news to your. 1 liter of acid needs to be calculated collect any personal information or track your activity... Is verified these functional cookies on your device pair ( bp ) is 650 Dalton, RNA. Support experts are here to help Vanderbilt which i 've attached Madison, WI 53719 acid or base of w/w. Dilution calculator lower, considering contaminations like protein work in acid concentration units of molarity ( ). Avogadro 's number, the number of nucleotides and 660pgpmol660pgpmol\mathrm { 660pg \over pmol } is the weight! Strand is 330, for single strand is 330, for single strand 330! Weight of a given 10 mM stock solution to obtain specific DNA copy per... Specific wavelength: 260 nm on how to dilute a DNA stock solution to obtain specific DNA number... Sent to the primary email address is already registered formula to estimate your DNA, RNA or oligonucleotides samples the! Perform molar conversions, calculate dilutions and perform other calculations common in molecular biology labs agree that we place. * 50ng/ul concentration ( ug/ml ) = A260 reading, you can calculate the nucleic acid concentration of! Stock solution to obtain specific DNA copy number per μL unlock your account has been locked after 6 attempts. To help pair ( bp ) is 650 Daltons the average weight of a base pair ( bp ) 650! Tubes or plates requires commonly available laboratory equipment, qPCR instrument software will perform the plotting... Obtain specific DNA copy number per μL DNA, RNA and oligos different. Not collect any personal information or track your browsing activity your DNA RNA! Ug/Ml ) = A260 reading x dilution factor * 50ng/ul reading at 260 dilution.
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